The study of enteritis vaccine

The study of enteritis vaccine
R. to (1962) has on the grass, black carp infectious enteritis disease for vaccine research, reported the artificial immunity method in laboratory injection, warm, cast in the pond feeding enteritis vaccine trials, and effects of environmental factors on vaccine and immune fish and control fish serum protein composition were determined. In experimental rabbit immune and pathogens of enteritis (punctata gas single cell bacteria) of preparation of vaccine, the rabbits in vivo stimulates the production of agglutinating antibody titer reached 1:5 160 grass carp mouth to feed and immunological test, rubber catheter for the vaccine was poured into the intestine and its concentration, with 100 million bacteria per milliliter, immune interval for 5 days, a total of three times, third feeding mouth vaccine after 7 days, attack tests. After feeding mouth vaccine, fish produced certain protective immunity in mice.
The pond fed immunity test, per kg body weight of fish fed 1 ml vaccine (25000000000 bacteria / ml), were fed 4 times. Although four pond to the end of December were not found in the diseased fish, but from the point of view of the fish in the moat attack test, after immune shows higher resistance. The three batch of fish, the first two batches of fish, the incidence of fish, although the number is equal to the control fish, but the time of death is later; the fish, the third batch of immune test, in addition to the onset time is relatively late, and the incidence of less than 20%, while the control group was as high as 80%.
Immune test of the gill disease of grass carp
Weihu harm inactivated vaccine and inactivated inactivated) in (and Ji Guoliang Chen Changfu 1988 of prepared three different fish harm Myxococcus Miao Miao formalin inactivated vaccine (adding 1% formalin, 28 DEG C for 24 hours, referred to as F-MP and phenol added 2% phenol, 28 degrees C for 24 hours (P-MP) and heat inactivated vaccine (bacteria liquid under 60 DEG C water bath inactivated 1 hour, referred to as H-MP) tested grass carp to 3 kinds of different fish Myxococcus vaccine immune response, the vaccine were diluted into a bacteria / mL concentration per tail muscle injection of 0.2 ml of immune fish. After immunization, the titer of serum antibody titer of the grass carp was 256 ~ 4 F-MP, 256 ~ 2 096H-MP, 128 ~ 1024, respectively. They were in the 21 to 28 days after the injection to reach the peak, 28 days after a gentle or slightly decreased. While the control group except for the individual antibody titer reached 8, the rest are in about 4. The mortality rates of F-MP.H-MP and P-Mp in the immunized group were 10%.16.7% and 5%, respectively, while the control group was 60%, which showed that the fish had a certain protective force.
In oral fish harm the viscosity of the bacterial lawn immunity test, in addition to the species of Lu vaccine, and fish harm sticky aureus ester polysaccharide (LPS), concentration of 3 kinds of vaccine were 109 bacteria / ml concentrations of LPS for 2 mg / ml, respectively according to the amount of per 100 tail fish every day 1 ml vaccine, mixed with bait in the early, late twice feeding. Attack test was carried out for 3 times, the first time in a row for 7 days, second times for 14 days, 21 times for third days. The results showed that the difference was not significant between the 1 consecutive days of attack and mortality. The 21 division was slow in death. The protective force was the beginning of feeding for 14 days.

Immune prevention and treatment of bacterial diseases

Immune prevention and treatment of bacterial diseases
In early 1942, Duff (Duff) began to cold water of silver carp and trout to fire frog Aeromonas bacteria immunity test, in our country the earliest is R. (1962) with the spot gas single cell bacteria brother shis control grass carp enteritis. From the 20th century? In the 1960s to now, prevention and control of bacterial fish disease vaccine has been a hot spot in the research of, but due to various reasons, the majority of domestic vaccine is still in research or production trial, has not yet reached the production of large-scale application stage.
The general process of vaccine preparation
1, we need to have the vaccine preparation of pathogenic strains of virulent, good antigenicity. Can a pathogenic strain, also can be more than two kinds of pathogenic strains combination preparation vaccine be Tuesday vaccine, triple vaccine,. 2, and train a large number of pathogenic bacteria. The suitable medium of solid medium or liquid medium was used to enlarge the culture medium at the optimum temperature to obtain the most bacteria. 3, the bacteria can be collected, and the culture of solid culture medium can be washed by sterile physiological saline, and then the bacteria can be collected directly by centrifugal precipitation. A certain concentration of bacterial suspension made with saline, which become viable han. Toxoid is bacterial exotoxins by 0,3% – 0.4% Faure Marin made. 4, will kill the live vaccine become inactivated vaccine. Destroy methods generally have o.1% ~ 0.5% formalin, or add 0.5% phenol, or plus 8% chloroform, placed in 25t 24 hours to inactivate, also can be used in heating method (60 ~ 651.2 hours or so) inactivation. The purpose of the inactivation is to kill the bacteria and to keep the complete disturbance as well as possible. Throw in the formalin test is more sensitive, with O, Wei% formalin killing live vaccine, if not remove formalin can cause the death of white crucian carp. 5, after the preparation of inactivated vaccine, to do sterility test, safety test, test titer test, can be used.