Immune prevention and treatment of bacterial diseases

Immune prevention and treatment of bacterial diseases
In early 1942, Duff (Duff) began to cold water of silver carp and trout to fire frog Aeromonas bacteria immunity test, in our country the earliest is R. (1962) with the spot gas single cell bacteria brother shis control grass carp enteritis. From the 20th century? In the 1960s to now, prevention and control of bacterial fish disease vaccine has been a hot spot in the research of, but due to various reasons, the majority of domestic vaccine is still in research or production trial, has not yet reached the production of large-scale application stage.
The general process of vaccine preparation
1, we need to have the vaccine preparation of pathogenic strains of virulent, good antigenicity. Can a pathogenic strain, also can be more than two kinds of pathogenic strains combination preparation vaccine be Tuesday vaccine, triple vaccine,. 2, and train a large number of pathogenic bacteria. The suitable medium of solid medium or liquid medium was used to enlarge the culture medium at the optimum temperature to obtain the most bacteria. 3, the bacteria can be collected, and the culture of solid culture medium can be washed by sterile physiological saline, and then the bacteria can be collected directly by centrifugal precipitation. A certain concentration of bacterial suspension made with saline, which become viable han. Toxoid is bacterial exotoxins by 0,3% – 0.4% Faure Marin made. 4, will kill the live vaccine become inactivated vaccine. Destroy methods generally have o.1% ~ 0.5% formalin, or add 0.5% phenol, or plus 8% chloroform, placed in 25t 24 hours to inactivate, also can be used in heating method (60 ~ 651.2 hours or so) inactivation. The purpose of the inactivation is to kill the bacteria and to keep the complete disturbance as well as possible. Throw in the formalin test is more sensitive, with O, Wei% formalin killing live vaccine, if not remove formalin can cause the death of white crucian carp. 5, after the preparation of inactivated vaccine, to do sterility test, safety test, test titer test, can be used.