Cell culture Grass Carp hemorrhage inactivated vaccine (the cell vaccine)

Cell culture Grass Carp hemorrhage inactivated vaccine (the cell vaccine)
Grass carp hemorrhagic tissue plasma inactivated vaccine, can enhance the immune ability of grass carp hemorrhage has popularized and applied in the whole country and achieved obvious effect. However, the production of this vaccine is derived from the diseased fish tissues, which is not stable, the yield is limited, and is not convenient for the factory production. Therefore, is currently being developed a kind of efficient, safe, easy to industrialized production of hemorrhage of grass carp, Ctenopharyngodon idella cell cultivation inactivated vaccine, will soon be published.
Cell death of live vaccine prepared first through cell culture screening strong virulence and immunity high grass carp hemorrhagic virus strains (strains currently used have ZV-8802.GCHV a 854), selection of GCHV sensitive cells (such as grass kiss end tissue strains ZC 7901, grass carp kidney cell line GCK a 84, grass carp CIK cells). With proper training method, the strain to adsorption method. Cell, until the cells just lesions appear, the virus cell with Hanks’ solution after washing, with 0.02% EDTA (EDTA) digestive fluid cell, then 10% calf serum 199 liquid made cell suspension, then add an appropriate amount of normal cells, to 25 DEG C under static culture, according to the method of continuous passage. To the tenth generation, the use of rotating bottle culture method to expand the cultivation. After the expansion of culture, the culture of 15 to 25 generation of virus was collected, which was used to prepare the inactivated vaccine. Inactivation methods by adding 0.1% formalin, 37 degrees C killed 48 hours to live or to 4 DEG C inactivation of 17 days and prepared cell inactivated vaccine to a safety test, and determination of the immune protection rate