Spring carp Viremia

Spring carp Viremia

Spring carp Viremia (also known as carp-catching ascites syndrome) is caused by a carp rhabdoviridae carp branch is an acute infectious disease, hemorrhagic. Systemic bleeding, ascites, characterized by the onset of acute and high mortality. OIE listing it as need to be declared to the OIE diseases.

1 the etiology

Spring carp Viremia virus, also known as carp rhabdoviridae, genus rhabdoviridae Vesiculovirus.

2 epidemiology

Infection as a sick fish, dead fish and poisoned fish. Transmission of water-borne virus via branchial and intestinal invasions. Possible vertical transmission, or by certain aquatic blood sucking parasites (Shi, inchworm, fish leech etc) mechanical transmission. Detox sick fish and poisoned the fish manure, urine, semen and eggs also contain viruses.

This disease mainly infected carp, carp of over 1. Silver carp and Bighead carp, crucian carp, Wels catfish in Europe can also be infected. Also enable the artificial infection of rainbow trout, grass carp, Northern Pike.

This disease are popular in Europe, the two continents, is the first class fish quarantine and quarantine objects. Main hazards 1 aged Cheng Li, to carp is most serious. Pop every spring (temperature 13~20℃), water temperatures over 22 ° c is no longer the disease, spring carp Viremia, hence the name.
Spring carp Viremia-clinical signs
The disease incubation period is 1-60 days.

Body color black, breathing difficulties, ataxia (side trip, drifting or swimming). Abdominal swelling, proptosis, anal redness, skin and Gill permeability and blood.

Anatomy characteristics of systemic bleeding edema and ascites. Gastrointestinal bleeding, accumulated within the abdominal cavity with serous or bloody ascites. Heart muscle, kidney, bladder, bleeding and inflammation, especially in the inner wall of the bladder are most common.
Spring carp Viremia-Diagnostics
1 according to the epidemiology, clinical signs and pathological changes may make a preliminary diagnosis, requires further laboratory confirmed the diagnosis.

2 laboratory Diagnostics

Enzyme-linked immunosorbent assay, direct fluorescent antibody test for virus detection, virus neutralization test.
Spring carp Viremia-combating
Use polyethylene of pyrrolidine iodine agent (PVP) mix the Fed can reduce mortality. Strict quarantine requirements water, the introduction of breeding fish eggs and fish virus. Discovered or suspected sick fish must be destroyed, fish culture facilities to be sterilized.

Common Viral fish diseases

Common Viral fish diseases
Grass carp hemorrhage (hemorrhage of grass carp) is most grass carp Fingerling rearing stage of the disease, as early as 1978, the hydrobiology Institute to study the pathogen of this report. , Chinese Academy of Sciences, Wuhan University, Wuhan Institute of Virology, CAFS, the Yangtze River Fisheries Research Institute in China, and many units have also conducted in-depth research to this pathogen. International grass carp hemorrhage virus found in China, and the first was reported. Research personnel on grass carp bleeding disease virus of form structure and physico-chemical characteristics, and virus of nucleic acid nature, and virus and host between of mutual role, and virus of organization pathology, and different virus separation strains of nature features, and virus of serum learn nature, and immune control, many carried out has research (Chen Yanxin,, 1983; Deng summer,, 1985; Min Shuqin,, 1986; Fang Qin,, 1989; Ke Lihua,, 1990; Ding Qingquan,, 1990; Jiang Yulin,, 1991a).

Pathogenic viruses for 20 solid particles, diameter 50~80nm, vary because of different isolates, such as Chen Yanxin, (1983) reported the virus particle diameter is 53nm; Ke Lihua et al (1990) reported the virus particle diameter is 71nm. No lipoprotein envelope of the virus, had a double capsid. Viral genomic nucleic acid is RNA, made up of 11 segments of total molecular weight of about 15×10-6. Virus in rostral cell line ZC7901 in grass carp, grass carp, cell line, CIK, GCK84, fish fin Minnow cell proliferation in CF84.

Epidemic disease and harm hemorrhage in FRY, viruses mainly carp, herring also makes artificial infection of diseases. With up to 2.5~15cm of fish disease is the most common, and often caused the death of a large number of grass carp fingerlings. 25 ℃ below fish is a latent infection, when water temperature exceeds 27 ℃, the disease can be widely popular, high mortality rates, often up to 90% or more. Fish virus infection can have different symptoms, such as “red muscle” symptoms as muscle blood red gills are often anemic and pale, this small fish found in 7~10cm; “Red fin red gills” symptoms as the fin base and the gills congestion, disease-prone in the larger fish (13cm) and “diarrhea” symptoms are characterized by intestinal serious congestion, small and large grass carp may occur. These three symptoms can occur alone or both.

Pathological changes in small blood vessels extensively damaged the wall, the formation of microthrombi and infarct-like lesions cause tissues and organs. In liver cells, such as intracytoplasmic Eosinophilic inclusion bodies can be seen. Percentage amount of white blood cells, lymphocytes, red blood cells and haemoglobin was significantly lower than fish, monocyte percentage is significantly higher than that of fish. Serum alanine aminotransferase, isocitrate dehydrogenase, lactate dehydrogenase activity increased plasma levels of total protein, cholesterol-lowering. Intestinal bleeding is the main symptom of sick fish to swelling of the intestinal epithelial cells, Goblet cells increases intestinal epithelium and lamina propria stripping, lumen has a large number of red blood cells and pieces fall off the intestinal epithelium.

Detection and prevention of fish Reovirus antiserum was prepared on the basis of, through discontinuous EP and enzyme-linked immunosorbent assay (ELISA) methods on early diagnosis of disease, but also by Staphylococcus protein a coagglutination test, histopathological and electron microscopic observation of the preliminary diagnosis of grass carp haemorrhage. Through the artificial immune system can effectively control grass carp haemorrhage. Tissue pulp inactivated vaccine and inactivated vaccines can be used in the production of cell culture practice, supplemented by using immune booster (such as hyoscyamine) can enhance the immune effect. Certain herbs, the rational use of antibiotics and, to some extent, slow the effects of the illness.

Referred to research on the virus disease of grass carp Reovirus outer in detail, there are other relevant reports. Mao Shujian (1989) isolated from grass carp in the pathogenesis of Zhejiang reported a small virus particles, diameter 20~30nm, genome as a single strand of RNA, no envelope, after the preliminary appraisal is a small RNA virus.
CARP virus
Viral disease of carp have been found and reported the main spring carp Viremia, carp Gill of smallpox disease, necrosis, and following a brief introduction, respectively.

Spring carp Viremia
Spring carp Viremia virus (Spring Viraemia of carp virus,SVCV) are pathogens that can cause acute infections in carp. After infection by the virus of carp in the pathogenesis of water temperature below 15 ° c in spring, and severe bladder inflammation symptoms are severe abdominal edema syndrome, and can often lead to secondary bacterial infections. Debate about the origin of the problem some, originally thought to be bacteria, until 1972 Fijan reported issued by the separation of infectious virus to be determined. SVCV genus rhabdoviridae family, this virus particles were rod and (or) bullet-shaped, long 135~380nm of mature virus particles, with a diameter of 45~95nm. Nuclear protein nucleocapsid consists of spiral wound (minus-strand single-stranded RNA and the n protein). Spring carp Viremia of outbreaks of water-related, and the optimum temperature for 16~17℃. Viruses can be spread by susceptible fish feces in the water, or some blood sucking parasites such as Lei Shi (Argulus Foliaceus) and inchworm fish leech (Piscicola geometrica Linnaeus) spread. In a State of nature, other fish besides carp, grass carp can also be infected. SVCV cells in some fishes such as FHM, effects of the EPC, and cause cell lesions. The pathogen by serological methods for diagnosis. May refer to several methods to control the SVCV: avoid the incoming virus source; Vigorously carry out drug cultivation of seedlings; Artificial control of water temperature (20 ℃ farmed fish); Immunization.
Necrosis of common carp Gill disease
Necrosis of common carp Gill (Gill necrosis of CARP) by Soviet scholars Shchelkunov and Shchelkunova (1981) reported that from a disease characterized by necrosis of Gill fish gills and kidney were isolated in virus, the virus can replicate FHM cells and infected fish disease. Preliminary judgment that the virus belongs to iridovirus.

CARP smallpox disease
Smallpox disease of carp (fish POX) smallpox disease is carp common diseases before and after winter, fashion is very broad. Main round belly Chub and carp, crucian carp, effects on fish growth and the value of goods. Fijan, (1983) isolated from diseased fish herpes virus (Herpesvirus). Jiang Yulin (1991b) reported the results of electron microscopic observation of carp smallpox pathogen. The typical symptoms of varying size in fish body surface is covered with massive white mucus, wax-like increase of biological, namely smallpox. These smallpox combined with the body surface of the fish is firm and can be scratched off with a knife. Remove smallpox into thin slices, electron microscope, there are a large number of virus particles present in the cytoplasm. Virus core 20 facets of Herpesviridae. Virus particle diameter 140~160nm, capsid diameter is 80~100nm. Virus nucleocapsid formed in the cell nucleus, get when the capsid bud through the nuclear membrane envelope, and gains the ability to infect cells. The viruses in epithelial cells of carp (EPC) cells, form a syncytium. After artificial infection test vaccinations to health carp virus suspensions in vivo, in 10~15℃ conditions 1 month later was get smallpox by inoculating the body surface of the fish. Pocked suspension with scratch after inoculation with Lei Yue Mun can be copied out in the laboratory with the same natural onset of symptoms, and by changing some of the environmental factors that control the occurrence of smallpox and disappeared. Under natural conditions, the virus can spread through mucous membrane exposure and contamination on the surface of the water. In addition to viruses, but Jeney (1995) on carp’s other diseases such as bacteria, fungi and parasites, a more comprehensive review of the progress, and control of fish diseases of great value.
Eel virus disease
Both at home and abroad have reported on eel virus, see the research.
Virus disease of eel type: virus of European eel (eel virus european,EVE), eel virus (eel virus,EV), eel Bush virus (cauliflower disease of eels virus), eel slug virus (rhabdovirus of eels), eel blisters virus (vesiculo virus ofeels). There is popular among other fish and infections viruses such as infectious pancreatic necrosis virus (infectious pancreatic nerosisvirus,IPNS), herpes viruses (herpeslike virus), Viral Hemorrhagic Septicemia virus (viral haemorrhagic septicaemia virus,VHSV) can also be infected eels (Castric, 1992).

Eel virus disease symptoms and pathogenic nature eel virus pathogen of the earlier study was EVE. Japan imported from European eel FRY in the 2nd year (1969), outbreaks of disease, and about half of eel deaths deaths a year in the same place only eels reach 2 700T. Only in 1973 on the eel from kidney disease viruses isolated. This double-stranded RNA virus of the genus eel virus pathogen, serology and infectious pancreatic necrosis virus (IPNV) close to the Ab strain, physical and chemical properties are identical to the IPNV. By EVE caused disease occurs mainly in winter, in Japan and China’s Taiwan caused Japan eel dead. 8~14℃, 4~26G 55% of the elver 20D mortality after infection. Main symptoms and pathological changes are: dying eel in temporary muscle spasms, or eels local rigid body, anal and some eels abdominal congestion, renal enlargement, ascites, Glomerular seriously damaged, some eels spleen necrosis of large areas. In naturally infected eels, Gill lamellae and hyperplasia of fusion phenomena (Huang Qiyan, 1993).

SchwantzPeitzner (1976) isolated from patients suffering from cutaneous Papilloma eel blood out of the EV. Viruses are round, with a diameter of 46~54nm. After viral inoculation of eels, do not produce any symptoms, but the virus particles in the cytoplasm of the host. Nagabayashi et al (1979) from the eel are ill isolated another virus in the blood, known as the eel Bush virus (cauliflower disease of eels virus). This virus, orthomyxovirus family (Orthomyxoviridae), type, diameter 90~140nm, 10nm long protrusions on the surface structure. Disease of eel head and lips clearly covered with hyperplasia of squamous cells, forming cauliflower-like head. Viruses are sensitive to ether, poisoning at 4 ℃ for 3 weeks, chickens and sheep red blood cell lectin. Virus on FHM cell culture cultivation temperature is 15 degrees Celsius.

In addition to the eels serological relationships of different isolates studied (Ahne, 1987). Jorgensen et al (1994) reported that scientists from several countries, such as 1977-1992 in Denmark and the United Kingdom, and France and the Sudan on 2 092 Man Chi virus test result. They got 91 virus samples, analysis showed that, of which 44 eel rhabdoviridae (eel rhabdovirus EVEX/EVA) genus Vesiculovirus (vesiculo group); 3 eel elastic-like virus belongs to the genus of rabies virus (Lyssa group); 38 infectious pancreatic necrosis virus (IPNS); 5 types of herpes virus, and Viral Hemorrhagic Septicemia virus 1. Visible, eel virus disease of harm exists and has caught the attention of scientists.

About the research profile of the first reports of eel eel virus virus disease is Chen Yanxin, (1992), suffering from a bloody opening of eel virus was found in blood cells. Virus particles 20 dodecahedral symmetry, consisting of a core and wrapped in film composition, particles with an average diameter of 70nm, inner core to 40nm. Mouth disease of eel in red blood cells and white blood cells, are seen under the electron microscope virus particles, but in the form of the virus found in the two size difference: virus 70nm in diameter in the red blood cells, capsule; In the white blood cells of diameter up to 40nm, not capsule. Fish is the main symptom mouth open not closed, in addition to sick fish is dark grey, head up, membrane bleeding in the mouth, and pectoral, pelvic and abdominal bleeding bit. Infected cells had abnormalities, such as wider or forming irregular folds of nuclear envelope; Round the swelling of mitochondria were vacuolated, and some contain Flocculent particles; Rough endoplasmic reticulum threshing and vacuolation. Recently, such as Tao Zengsi (1997) reported on eel “swim” pathogens research results show that the disease is caused by the “coronavirus-like virus” causes. Their condition is a disease of eel down in the water ran, spin crazy tour, muscle cramps, chest bruises; At necropsy liver enlargement. Histopathological examination showed liver, kidney and nervous cell degeneration and necrosis of the heart.
Viral diseases of rainbow trout
Rainbow Trout for cold water fish (cold water fishes), natural distribution in the United States, and Canada, and Mexico and other places. Trout began in 1959, but since intensive farming in 1983 from the United States, and Japan began only after the introduction of fine varieties of trout. Has been studied in detail in several important virus diseases of fish, at least three serious threats to farming of rainbow trout, which is infectious pancreatic necrosis (infectiouspanereatic necrosis,IPN), Viral Hemorrhagic Septicemia (viral haemorragic septicemia,VHS) and infectious hematopoietic necrosis disease (infectious hematopoietic necrosis,IHN)。

Infectious pancreatic necrosis disease
Rainbow Trout research on infectious pancreatic necrosis and viral pathogens is the Wolf (1959) began after reports this is a viral disease. Wolf (1988a) reviewed more than 200 articles in the literature based on the history of study of infectious pancreatic necrosis virus of fish, host and geographic distributions of, physicochemical properties, separation, identification, immunity and control aspects are described in detail. Since this virus serious, popular, as a first class national fish quarantine and quarantine objects. Main symptoms of infectious pancreatic necrosis is perpendicular to the fish swim, fish blackened, proptosis, swelling of the abdomen, anus and often drag cable-like mucus. Infectious pancreatic necrosis virus (IPNV) sensitive many fish, such as some varieties of salmon, trout, salmon, mainly against day-old FRY and 14-17 species, mortality rates of up to 80%~100% when the water temperature 10~12℃; Other fish from 20 families also in detecting IPNV and most poisonous fish illness did not appear. Studies have shown that, IPNV levels can not only spread poison led to vertical transmission through the egg. The most visible pathological changes of pancreatic necrosis, cell nuclear pyknosis and fractured, some of the inclusions in the cytoplasm of the cell. IPNV belongs to the double-stranded RNA viruses, with 20 grain, about 60nm in diameter, there are RNA-dependent RNA transcriptase. IPNV RTG2, R1, PG and CHSE cold-water fish cell lines cell proliferation and caused varying degrees of lesions. In Rainbow Trout imported in 1987 from the isolation of IPNV, and serological identification of IPNV rapid detection method of rainbow trout was also established (Jiang Yulin, 1989, 1990).

Viral Hemorrhagic Septicemia
Viral Hemorrhagic Septicemia virus (VHSV) is a bullet-shaped virus, can cause acute and subacute infection of rainbow trout mortality as high as 80%. Histopathological characteristics of the hematopoietic organs such as the spleen, kidney, and pancreatic necrosis. Acute type characterized by proptosis, bleeding and rapid death; Chronic infection characterized by slow movement disorders, anemia, and death. Epidemic in late winter and early spring, water temperatures below 14 c during outbreaks. Pathogenic diagnosis can be formed by taking the sick fish entrails, blood, or ascites for virus isolation, inoculated tissue homogenate or body fluids of sick fish to RTG2, CHSE, FHM, EPC or PG cells, 15 training 1~2D lesions can occur, complemented by neutralization test or fluorescent antibody technique can be used for isolation of strains for identification.

Infectious hematopoietic necrosis disease
Infectious hematopoietic necrosis virus (IHNV) rhabdoviridae, Huang Qiyan (1993) on the virus and the disease has been introduced. Infectious hematopoietic necrosis disease initially in the United States occurred, found in North America and Japan as well. Zhao Zhizhuang et al (1993), reported that the China’s Benxi rainbow trout virus IHNVB separation of hybridoma cell lines, monoclonal antibodies and ELISA detection method. Leng (1995) wrote that with IHNV from broodstock to eggs or sperm can be virus progeny, and a descendant of fish can survive a few individuals, these fish survive once it can no longer be IHNV infections cause disease, electron microscopy and molecular techniques (PCR) showed that, in those still surviving fish can be found in the presence of RNA of IHNV. Why the surviving fish disease but also against IHNV infection? Drolet et al (1995) studies have solved the mystery: the original flawed virus particles in these fish (DI), and DI for IHNV interfere with infectious, which shows the fish a molecular mechanism of resistance to viral infections, also explore the treatment of fish disease provides a new way of thinking. In addition in recent years has reported on other viral diseases of rainbow trout (Nylund, and others, 1995).
Mandarin fish siniperca chuatsi virus edit this paragraph
The Mandarin fish siniperca chuatsi [Siniperca chuats (Basilewsky)] virus abroad about the Mandarin fish siniperca chuatsi virus disease research are yet to see, but in recent years the Mandarin fish siniperca chuatsi diseases in Guangdong and other places popular with serious, illnesses mainly Mandarin fish skin bleeding point, Gill pale; Anatomical visible damage caused by a lot of bleeding, liver, spleen, gall bladder and bowel congestion and significantly enlarged. And harm of the disease in 1993, occurred only in a small number of farms, but extremely fierce, against the 1994-1995 of the same diseases, and in the Pearl River Delta is widespread. Incidence of tens of thousands of hectares of fish ponds 60%, serious disease of fish mortality than 90%. Use medications such as antibiotics, to no avail; Bacteria pathogenic bacteria could not be found. Researchers at the Institute of hydrobiology of the Mandarin fish siniperca chuatsi in fulminant epidemic of liver, spleen and heart tissue thin section electron microscopy, found in the cytoplasm, nucleus and cell gap distribution there are viral particles in. Virus morphology is spherical, its diameter is about to 280nm. Filtration and degerming crude after virus vaccination to fish cells in monolayer culture can make cells lesion; Healthy sauce using crude extract of virus or amplification of the virus infection in cultured cells, could cause the morbidity and mortality of the Mandarin fish siniperca chuatsi. Mandarin fish siniperca chuatsi on health (both artificial and natural infection of fish) were dissected, and the gills, liver, spleen, heart and renal histopathological observation 5 organs, confirmed the 5 organs of the Mandarin fish siniperca chuatsi diseases have taken place, and artificial infection of fish and fish in the pathogenesis of disease in natural state consistent with the pathological condition. These results show that of Mandarin fish siniperca chuatsi viruses discovered by (siniperca chuatsi virus,SCV) is the Mandarin fish siniperca chuatsi fulminant epidemic disease (such as Zhang qiya, 1996a).
Spotted catfish channel catfish virus disease
Spotted cross channel catfish virus disease (channelcatfishvirusdisease,CCVD) spotted catfish channel catfish virus (CCV) is a herpes virus, which can cause spots cross channel catfish (IctaluruspunctatusRafinesque) FRY and young fish acute hemorrhagic diseases, mortality is as high as 100%. Fijan, (1970) reported the first results of CCV. Wolf (1988b) study reviews the progress of the CCV, introduced the history of CCV, infection caused by illnesses and pathological changes, physical and chemical properties, diagnosis and isolation, such as immunization and prevention. CCV as spherical particles, the capsule of a virus particle diameter 175~200nm, capsid diameter of about 100nm, genome for double-stranded DNA. CCVD outbreak is related to water temperature and fish age, when water temperature at 25 ℃, when the fish susceptible to, the incubation period is only 7~10d and acute onset. Disease characterized by uncoordinated spiral swim fins and bleeding at the base of the tail. Anatomical visible disease of kidney, spleen enlargement, internal bleeding, and pathological examination and hematopoietic necrosis, nuclear pyknosis and vacuolation. Spotted catfish channel catfish farming in some parts in China has taken shape, with the promotion of intensive farming techniques, more and more serious diseases, to establish awareness is very important. Serological and molecular biological diagnostic techniques have been successfully used in diagnosis of CCV (Wise, 1985), application for reference. Also worthy of special mention is the world’s first virus recombinant gene and its expression in fishes have been successful (such as Zhang, 1996), which is aimed at the CCV made an important study. This development heralded hopes to use genetically engineered vaccines to prevent viral diseases of fish.

Common fish diseases control

Common fish diseases control

Fish life in the water of life, and environmental factors is complex, often hit by viruses, bacteria and parasites, so fish is sick for many reasons, often cause large numbers of deaths after the fish is sick, even if the fish is sick is not death but also seriously affected the growth of fish. Like other animals do not treat fish diseases and treatment, have difficulties in water treatment and isolation. In aquaculture, therefore prevention is an important measure to prevent fish disease, prevention is more important than cure.
First, the prevention of fish disease
Prevention of fish disease from three aspects, one is to destroy pathogens and predators, second is to improve the living conditions of fish, making the environment conducive to the growth and health of the fish, the third is enhanced resistance of the fish.
(A) the clear pond
Put pond water dry or drain, clearing the pond bottom and Tong Geng, trash, weeds, fix Geng feet to prevent water seepage, leakage, keep the pond bottom mud 10cm, Tang 5-7 at the end of the day, sunlight exposure, fish pond, pathogens and predators for biological removal of more complete.
(B) the fish pond sterilization
1. Health lime disinfection: ponds clearing Hou, in dry pool conditions Xia with health lime 50-75 kg/acres, full pool spilled, health lime with wood barrels or plastic barrels, container Sheng water dissolved Hou, hot spilled, as bottom also left 5-10cm of water, is can without container, in pool end of uniform of dug many shallow pit, will health lime directly put to each small pit,, sucking hydration open Hou, will lime water spilled full pool, again with rake will pit within of lime rake moving mix. 1-2 days can be basal, 2-3 days after fresh water, 5-6 days can fish.
Another is water disinfection methods, such as fish ponds and drainage problems or anxious when species, this method can be used, depth of 1 m per acre quicklime 130-150 kg, evenly sprinkle it in the whole pond, 7-8 days after the fish.
2. Bleach disinfection. After draining the pond water with bleaching powder 4-5 kg/acre, water sprinkle it in the whole pond, amount of bleach with water to a depth of 1 m per acre 13-15 kg, 5-6 days after fish, note that bleach does not use a metal container holding water dissolves. Amount of chlorine bleach in about 30%, that pungent smell bleach large, insoluble in water heating.
3. Tea disinfection. Average water depth of 1 m per acre when dosage is 40-50 kilograms, tea cake is first crushed into small particles, is immersed in the solution for a day and a night, even slag with juice evenly sprinkle the whole pool, tea cake can kill wild fish eggs, tadpoles, snails, frogs and aquatic insects, but not do much help to kill bacteria and parasites.
Three Qing Tang disinfectants, lime and chlorine bleach has a number of advantages, kill bacteria more thoroughly, and lime as well as improving water quality.
(C) species disinfection
Species will inevitably bring some pathogens, after disinfection of pond clear, with no stocking of fingerlings disinfection will bring pathogens to the water, once the conditions are right, multiply and cause disease. Disinfection drug dipping method is often used for species.
1. Bleaching powder and copper sulphate mixture for dipping. 1 cubic meter water with bleaching powder 10 g and 8 g copper sulfate. Note: bleaching powder and copper sulphate after dissolving contact separately by different blending mixture, dipping fish in the 10-20 c 20-30 minutes.
2. Potassium permanganate solution dipping. 1 cubic meter of water used 20 grams of potassium permanganate, when dipping the fish 10-20 1-2 hour, liquid preparation used, over a long period of failure.
3. Dipping salt solution. With sputter the salt solution, 10-20 dipped Fingerling 4-5 minutes.
Any solution when dipping fish, dipping the length of time appropriate to the species healthy and low temperatures to make appropriate adjustments.
(D) water disinfected on a regular basis
Most fish disease of occurred, are has must of seasonal, many fish disease in 4-October popular, especially 6-September, water temperature increased, fish growth speed speed up, disease original body and predators biological large breeding, fish disease also easy occurred, so strengthening farming water of feeding management, insisted regularly water drug disinfection, improved water environment, is prevention fish disease occurred of important measures.
1. In April, and in late May to mid June, monthly 90% crystals of dipterex on aquaculture water disinfection to kill parasites, dosage is 0.5 grams per cubic meter, surface 1 m depth for 6-7 two per acre. 1-2 acres of fish ponds of small bodies of water, and sprinkle it in the whole pond methods available, large surface cannot be splashed all over the whole pool of cases, methods that can be used near food bags hanging bag, hanging 3-6, 100-150 grams per bag charge for 2-3.
2. June early with bleach or health lime on water disinfection sterilization, bleach dosage for 1 cubic metres 1 grams, dissolved Yu water full pool spilled, larger water can used hanging basket method, each only baskets within loaded drug 100-150 grams, hanging 3-6 only basket, hanging in food field near, or with health lime on water sterilization disinfection each acres water depth 1 meters dosage for 15-25 kg, dissolved water full pool spilled, larger water with health lime can in food field around slightly poured reuse volume, water other place to as spilled to.
From late June to early July with copper sulphate and ferrous sulphate mixture to sprinkle it in the whole pond, Protozoan parasite-killing, prevent the occurrence of protozoal disease, the dosage of 1 cubic meter 0.7 grams, which is 5:2, or bagging method, each containing 100 g, 40 g of ferrous sulfate copper sulfate, General hung 3-6 bags.
3. From July to September with the increase of water temperature bacteria multiply, are prone to diseases and, therefore, every 20-30 days applying bleaching powder or lime pool splashing or bags hanging basket method of killing pathogens, the prevention of fish disease.
Second, the diagnosis and treatment of several common fish diseases
1. Red grass carp disease
Red skin disease is by fluorescent very hair Bacillus invaded for netting and the handling in the skin injured of fish body and caused, main symptoms is: fish body local or big Department congestive, and inflammation, and scales off, especially fish body sides and the abdominal most obviously, part or all fin article of base Department congestive, fin article shoots end rot, fish of Shang jaw and the Gill cover part congestive, rendering block erythema.
The disease can occur from early spring to winter, but are more common in late spring to early autumn, the main harm grass carp.
Treatment methods:
(1) the bleach: water treatment 1 gram per cubic metre, water sprinkle it in the whole pond.
(2) five times: 2-4 grams per cubic metre of water treatment, five times a mash with boiled water for 1-2 hours, whether residue or juice to sprinkle it in the whole pond.
(3) the bacteria with the exception of the disease outside the skin, muscles, causing lesions in fish, will invade the blood of fish, except for drugs, must also be used for internal use, in accordance with the description of the medicine used.
2. Gill-rot disease
Fish Gill-rot disease is caused by damage caused by Neisseria mucosa into the gills of a fish, the main symptoms are: sick fish often stray du tour, slow, loss of appetite, or not eating, body color black, especially on the head is black, so it is also called “Wu-blast.” Fish Gill rot disease white, often accompanied by sludge and slime, and severe congestion on the inner surface of the operculum, the middle portion of the skin is often eroded into a small transparent window, also called the “open window”.
This disease is often found in the grass, herring, Bighead carp, carp and other fish, mainly carp, 4-10 months the water temperature 20 deg in fashion in General, often with red skin, enteritis diseases in grass carp at the same time.
Treatment methods:
(1) lime sprinkle it in the whole pond, depth of 1 m per acre, is 15-20 kg.
(2) bleaching powder sprinkle it in the whole pond, water 1 gram per cubic metre.
(3) five times child sprinkle it in the whole pond, 2-4 grams per cubic metre of water treatment, warm water immersion, whether residue or juice spilled.
(4) birds with erythromycin and sprinkle it in the whole pond, 0.3 grams per cubic metre of water medication the next day, mix feed will this medicine taken orally, by 100 kilograms of fish on the first day for 4 g, the second through sixth days halved.
(5) water depth of 1 m per acre with 2 kg rhubarb, 0.3% ammonia-soaked for 10 hours, sprinkle it in the whole pond, rhubarb-ammonia ratio is 1:20.
3. Enteritis diseases
This disease is caused by intestinal gas production a single Spore, and the main symptoms: fish, loss of appetite, slow, from the single swim, abdominal swelling, Erythema, swelling, the anus, and tap in the abdomen, anus and a light yellow mucous outflow, intestinal inflammation, hyperemia. Often associated with the Gill-rot disease of grass carp at the same time.
Season May-September, major hazard 1-2 carp, water temperature is 20 degrees or more.
Treatment methods:
Treated with oral and topical medication in conjunction. For external use 1 gram per cubic meter water used bleach spilled over the whole pool or the quicklime sprinkled over the pool, water depth of 1 m is 15-20 kg per MU.
Medicine medicine can be purchased according to the directions, the other herbs that are available are as follows:
(1) garlic: per 100 kg of fish with garlic 0.5 gram (mash and blend in the flour or rice flour) and 200 grams of salt per 5 kg of feed, mix well for 3 days with fresh grass, once a day.
(2) to acalypha: hay per 100 kg of fish of 0.5 g or 2 kg fresh grass mixed inside the bran, flour, feed, for 3 consecutive days, one day at a time.
(3) hydropiper and acalypha: hydropiper per 100 kg of fish and acalypha 1 kg mixed with water 5 kg, boiling 1-2 hour, after cooling, mix feed, continuous feed 3 days, one day at a time.
These three kinds of bacterial fish diseases, often complicated and prone to infection and, therefore, prevention and treatment of fish disease and fish disease prevention should be emphasized.
4. Viral haemorrhagic disease
Hemorrhagic disease is mainly caused by herpes virus, characterized by three types of symptoms:
1. “Red muscle” type: this type is more common in the smaller grass species, fish had no significant bleeding or only minor bleeding, but grilled in addition to skin, muscles visible point or massive congestion, when muscles are bright red, Gill was port-congestion or gills appear “white gills.”
2. “Red fin red operculum” type: fish’s operculum, fin base, head, eyes, mouth and other significant congestion, sometimes scales also have congestion, but muscle congestion is not obvious. Mainly in the large grass carp has come.
3. “Colitis”: the fish skin and muscle pump is not obvious, but cut intestinal visible in whole or in part with a bright-red, mesenteric sometimes dotted congestion, and enteritis of distinction, not a mesentery, intestinal inflammation, ulcers.
Treatment methods:
(1) per acre of water depth of 1 m by 0.5 kg rhubarb, grind into fine powder, fried boiled or soaked in water overnight, mixed feed and bait, and continued for 5 days, then 0.7 grams of water per cubic metre sprinkle it in the whole pond with copper sulphate.
(2) water depth of 1 m per acre, with rhubarb, Cork 0.5 kg 0.5 kg, scutellaria 0.5 kg, Berberine, salt 0.1 kg 2.5 kg, 10 kg of lime, mixed pulp and sprinkle it in the whole pond.
(3) drugs that can be purchased to make a good, used according to the instructions.

1. “Red muscle” type: this type is more common in the smaller grass species, fish had no significant bleeding or only minor bleeding, but grilled in addition to skin, muscles visible point or massive congestion, when muscles are bright red, Gill was port-congestion or gills appear “white gills.”
2. “Red fin red operculum” type: fish’s operculum, fin base, head, eyes, mouth and other significant congestion, sometimes scales also have congestion, but muscle congestion is not obvious. Mainly in the large grass carp has come.
3. “Colitis”: the fish skin and muscle pump is not obvious, but cut intestinal visible in whole or in part with a bright-red, mesenteric sometimes dotted congestion, and enteritis of distinction, not a mesentery, intestinal inflammation, ulcers.
Treatment methods:
(1) per acre of water depth of 1 m by 0.5 kg rhubarb, grind into fine powder, fried boiled or soaked in water overnight, mixed feed and bait, and continued for 5 days, then 0.7 grams of water per cubic metre sprinkle it in the whole pond with copper sulphate.
(2) water depth of 1 m per acre, with rhubarb, Cork 0.5 kg 0.5 kg, scutellaria 0.5 kg, Berberine, salt 0.1 kg 2.5 kg, 10 kg of lime, mixed pulp and sprinkle it in the whole pond.
(3) drugs that can be purchased to make a good, used according to the instructions.

Principle of using antibacterial drugs

Principle of using antibacterial drugs
(1) the correct diagnosis of the cause, rational use of drugs, strict control of the drug’s indications
Correct diagnosis is the primary condition for successful treatment. The main infectious diseases of fish bacterial diseases, viral diseases. Parasite disease called invasive disease. It is easy to diagnose the disease. It can be found that there is a large number of pathogens by the naked eye or by the microscope. According to the general symptoms of infectious diseases of fish and combined with laboratory tests to determine, but in practice, if we can exclude the parasite can use broad-spectrum antibacterial drugs for diagnostic treatment. After the observation for several days, if the effect is not obvious, it should be considered whether the virus disease, or re adjust the treatment plan. Should pay attention to the need for a certain period of time to play the drug, can not expect the day of the drug can quickly bear fruit. In fact, at one or two days after treatment, there is often a phenomenon of increasing mortality. This may be due to drug pathogenic bacteria in fish kills, prompting bacterial cells and release of endotoxin, acute poisoning caused by the death of the fish, this case is generally 3 – 4 days mortality will decrease, otherwise you should consider whether the drug overdose causes.
In addition, there is often a mixed infection of multiple pathogens in the late course of the disease. The common clinical car caused by grass carp gill rotifers and mixed bacterial infection, grass carp reovirus and gas production of single cell infection caused by enteritis, then there must be treatment options for different pathogens.
The correct diagnosis, selection of drugs according to drug indication, and adopt appropriate methods of administration.
(2) drug use time sooner rather than later fish once the disease, the symptoms appear loss of appetite, not feeding bait, oral drug on it can not afford to use fish in the pond after onset of action why drugs can control the disease? That’s because the drugs that are used to prevent the fish from the onset of the disease have a protective effect, so the real treatment for fish is not. It also shows that the more importance in fish disease prevention measures in yi.
(3) it is important to emphasize the importance of the immune system in the treatment of the disease. It is necessary to realize the importance of the immunity of the fish body.
(4) oral and topical use of drugs, external use of drugs is to play a role in the treatment of drugs on the surface of the body, and oral medication is to play its role in the absorption to control systemic infection. For bacterial diseases, whether it is a surface infection or systemic infection should be taken orally, external use of combination. For the infection of parasites in the body surface can be used only for external use, but sometimes it can also be effective for oral administration.
(5) to observe the effect of treatment in time, if the treatment effect is not obvious, we should analyze the reasons from the aspects of diagnosis, medicine, dosage, administration method, etc.. But should not be too frequent replacement of the drug; using a treatment regimen should be at least 72 hours after the observation to determine its efficacy, otherwise it will only result in drug abuse.
(6) to avoid the generation of bacterial resistance, which requires adequate dosage and treatment, and to pay attention to the use of different antimicrobial agents. Parasite resistance to drugs is also common.

Basic principles of drug use

Basic principles of drug use

Effect of judgment
Drug treatment can be judged from the following aspects: 1, to alleviate the symptoms and reduce the number of deaths. 2, food consumption increased. 3, normal swimming behavior. 4, body-color back to normal.
If antimicrobial treatment failure should be viewed against the following aspects and make medication adjustments: 1, incorrect diagnosis, pathogens are not clear, cause unclear and improper drug selection, 2, although accurate, but due to take chances, delayed the timing, because it’s too serious, most of the fish loss of appetite can not take bait. 3, drug problems, such as lack of effective drugs, or drugs not, or an arithmetical error, or dosing the right way, or using inadequate doses and treatments are not long enough, unable to control infection or cause relapse. 4, no elimination of sources of infection or cause is not eradicated, 5, remove any move sick fish, ponds, removal of stress factors such as illness, an increase in deaths. 6, the body’s defenses lower or disappear, 7, pathogenic bacteria develop resistance. 8, had dual infections. 9, physical and chemical characteristics of seawater and freshwater different, for many drugs (such as copper sulfate, oxytetracycline) efficacy have a great impact, required special attention.

Drug efficacy depends mainly on drug concentration in the blood and its duration, drug concentration in the blood only reaches the minimum inhibitory concentration (the minimum drug concentration required to inhibit the growth of bacteria) or more can play effect. Fish drug concentration in drug formulation, varies with the dose and method of administration, and the species of fish-related. Even the same species, but also because of its size, water temperature, food consumption varies depending on the conditions. In addition, the antibiotic concentrations in the blood are valid, should be based on susceptibility of pathogens. If pathogens are highly sensitive to this drug, the dosage can be less blood levels can be low 1 such as bacteria are only moderately sensitive, in terms of the quantity and blood levels must be high ¥ if the bacteria is not sensitive to drugs, strong resistance, even if the dose is large, nor to inhibit or kill bacteria.